Composite

Part:BBa_K2680106:Design

Designed by: Xiangyi Fang   Group: iGEM18_William_and_Mary   (2018-08-01)


3G pLacCIDAR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 263


Design Notes

Sequence from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary. "LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs."1

Source

"The Plac insert was PCR'd from the MG1655 strain of E.coli K12."1

References

1Part:BBa_R0010. (2003, January 31). Retrieved October 17, 2018, from https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010